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An improved micro-scale protein precipitation procedure for HPLC assay of therapeutic drugs in serum
Summary
Type:
HPLC
Application #:
104280
Catalog#:
720019.40 - MN HPLC Column, Nucleosil Phenyl, 100A, 7um, 4x250mm
Detection:
UV, 210nm
Conditions:
eluent: 30% acetonitrile in 0.02M phosphate buffer pH 7
flow rate 1.3ml/min
Authors:
Lam S
Source:
J Liquid Chromatogr 12 (1989) 1851 - 1872
Details
Legend: Fig. 8. Chromatograms of A) serum standard with 500ng/ml pyrimethamine and B) patient serum with 207ng/ml pyrimethamine, 100µl serum specimen deproteinized with 10µl aqueous zinc sulphate (10% w/v)
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